O-272 Oral administration of antioxidants can support follicle survival in xenografted human ovarian tissue

Abstract Study question Can oral administration of antioxidants improve follicle survival in human ovarian tissue through reduction oxidative stress a xenotransplantation model? Summary answer Daily for 7 days post-transplantation had positive effect on grafted tissue. What is known already Today cryopreservation and autotransplantation an established fertility preservation method many centres. Hundreds live births have been achieved but the efficacy this procedure hampered by critical loss follicles following transplantation. The subjected to hypoxia ischemia postoperative which leads damage Postoperative supplementation with has shown decrease transplanted increase survival, however, these results were mainly obtained intraperitoneal injections host animals. Oral could be better alternative clinical application. design, size, duration Pieces frozen-thawed cortex (total 56) from 12 women immunodeficient mice short- long-term xenograft study, 28 respectively. In each study administered daily doses hazelnut cream (control group) or containing either 150 mg/kg N-acetylcysteine (NAC) FERTILIX (containing Vitamin E, D C, Mixed Tocopherols Tocotrienols, Lycopene, Coenzyme Q10). Participants/materials, setting, methods Cryopreserved was donated undergoing at Copenhagen University Hospital. Grafts retrieved after xenografting histologically processed counts, immunohistochemistry, qPCR analysis. number surviving graft volume evaluated xenografting. Gene expression analysis antioxidant defence markers (Sod1, Hmox Catalase) angiogenic factor Vegfa performed Main role chance After xenografting, density similar all groups group: 33.03±14.85 (mean±SEM) follicles/mm3; NAC-group: 26.34±10.54 follicles/mm3, FERTILIX-group: 35.12±10.88 follicles/mm3). However, NAC- FERTILIX-group 3- 3.7-fold respectively compared control group. there higher both groups, 9.51±2.9 14.9±6.02 14.43±5.83 follicles/mm3), resulted 3.2-fold NAC-group 2.4-fold findings not statistically significantly different (p = 0.7072; p 0.5691). relative gene genes Catalase overall like treatment Sod1 lower group 0.0407; 0.0255). For > 0.05). Limitations, reasons caution distribution highly heterogeneous can vary tremendously between pieces same woman patients. Statistically significant data current study; thus, conclusions should interpreted caution. Wider implications xenotransplanted These provide step toward potential NAC transplantation, outcomes feasible way patient. Trial registration Not applicable